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The purpose of the present study was to assess immediate DNA damage after exposure to low level of ionizing radiation by the flow cytometric method of gamma-H2AX. Here, measurements of γ-H2AX immunofluorescence by microscopy and flow cytometry were compared as rapid biodosimetric tools for whole and partial body exposures. View This Abstract Online; Flow cytometric detection of gamma-H2AX to evaluate DNA damage by low dose diagnostic irradiation. Med Hypotheses.

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These results are further supported by the direct comparison of flow cytometry data and integral fluorescence intensity as extracted from microscopy pictures which is shown in S1 Fig . 6 gamma-H2AX Primary Antibodies: Thermo Fisher antibodies are validated for applications including western blotting, immunocytochemistry, flow cytometry, and chromatin immunoprecipitation. Histone H2A.X (H2AX) is a member of the histone H2A family which is one of the four core histones making up the nucleosome core particle. In eukaryotes, DNA double strand breaks (DSBs) have been shown to trigger the phosphorylation of serine 139 at the carboxy terminus of histone H2AX resulting in gamma-H2AX.

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In this chapter, we provide our experience and methodological modification of flow cytometry protocol for the detection of γ-H2AX, a well-known marker of DSBs, in fixed mammalian fibroblasts. The purpose of the present study was to assess immediate DNA damage after exposure to low level of ionizing radiation by the flow cytometric method of gamma-H2AX.

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H2AX phosphorylation at the SQ motif (γ-H2AX) has been Furthermore, by using DIM, flow cytometry, immunoblotting, and quantitative imaging microscopy   25 Jul 2017 γ-rays. DSB were enumerated with γH2AX foci using imaging flow cytometry. Phosphorylated form of histone H2AX (γH2AX) is a generally accepted UCB MNC were irradiated with γ-rays (0, 5, 10, and 50 cGy) and then& Clone REA502 recognizes the human and mouse histone H2AX antigen phosphorylated at serine 139 (pS139). X, γ-H2AX, γH2AX Intracellular flow cytometry (3) applications for H2AX pS139 Antibody, anti-human/mouse, REAfinity™.

Product View. Your search returned 63 H2AX Flow Cytometry Antibodies across 9 suppliers. Showing 9 of 9 suppliers (63 products total) <<. Histone H2AX: Products. Histone H2AX is one of a number of core histone proteins. In the cellular response to genotoxic insults, ATM and related protein kinases phosphorylate the carboxyl-terminal tail of the H2AX protein (gamma-H2AX). gamma-H2AX marks the site of damage and provides a nucleation site for the formation of damage response and repair complexes.
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Artikel i vetenskaplig tidskrift, refereegranskad. A control for the day-to-day normalization of the flow cytometry γ-H2AX assay for clinical routine. Artikel i vetenskaplig tidskrift, refereegranskad.

assays for the sensitive measurement of g-H2AX, using both microscopy and flow cytometry (21–25). Analysis of g-H2AX by microscopy is still considered to be the most sensitive detection method and may discriminate between differential g-H2AX responses with respect to drug type and cell population makeup (7). However, the In contrast, flow cytometry allows simple detection of gamma-H2AX in a large number of cells .
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The best sensitivity is achieved by computer analysis. Using immunofluorescence and imaging flow cytometry, foci were measured in untreated cells and at 0.5, 3, 5 and 24 hours post-irradiation. In all lymphoblastoid cells treated with 2 Gy gamma radiation, there was a predictable induction of DNA strand breaks, with a modest but significant retention of foci over 24 hours in irradiated cells treated with Olaparib (ANOVA P 0.05). flow cytometry (1) immunocytochemistry (2) ELISA (2) dot blot (1) Host Species. mouse (1) Species Reactivity. Anti-Gamma H2AX (phospho-Ser139) antibody, Rabbit H2AX, H2a.x, H2afx, H2A/X 89 citations have been found for this product All applications Flow cytometry/Cell sorting (FC/FACS) Immunocytochemistry (ICC) Immunocytochemistry-immunoflourescence (ICC-IF) Immunofluorescence (IF) Immunohistochemistry (IHC) Immunohistochemistry-immunofluorescence (IHC-IF) Immunohistochemistry-paraffin (IHC-P) Immunoprecipitation (IP) Western Blotting (WB) Anti-gamma H2A.X (phospho S139) antibody [EP854(2)Y] - BSA and Azide free Abcam catalog: ab215967 BioAssay record AID 1254631 submitted by ChEMBL: Induction of DNA damage in human HCT116 cells at G0/G1 phase at 30 uM after 24 hrs by gamma-H2AX-staining based-flow cytometry. Additional reported applications (for the relevant formats of this clone) include: immunohistochemistry on paraffin embedded sections 2, immunofluorescence microscopy 3-9, Western blotting 10-12, and flow cytometry 1,13.