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The fluorescence of EtBr increases 21-fold upon binding to double-stranded RNA and 25-fold on binding double-stranded DNA so that destaining the background is not necessary with a low stain concentration (10 μg/ml). A trypanocidal agent and possible antiviral agent that is widely used in experimental cell biology and biochemistry. Ethidium has several experimentally useful properties including binding to nucleic acids, noncompetitive inhibition of nicotinic acetylcholine receptors, and fluorescence among others. It is most commonly used as the bromide. Ethidium bromide is a sensitive fluorescent stain for visualizing DNA in agarose and polyacrylamide gels.

Ethidium bromide

Components: H504. Ethidium Bromide Solution ethidium bromide when viewed with the naked eye, but sensitivity is the same as ethidium bromide. (better signal:noise ratio), when. (c) Results of the PCR shall be assessed following electrophoresis using a 2 % agarose gel stained with ethidium bromide and including size markers alongside Ethidium Bromide Solution.

SÄKERHETSDATABLAD - Thermo Fisher Scientific

Ethidium bromide is not a regulated hazardous material, but due to its potential health hazards, precautions should be taken during handling and disposal. Ethidium bromide gels, stock solutions, buffers and contaminated debris should be referred to EHS for collection and disposal. The mechanism of the enhancement of the fluorescence of ethidium bromide on binding to double helical RNA and DNA has been investigated.

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The fluorescence of EtBr increases 21-fold upon binding to double-stranded RNA and 25-fold on binding double-stranded DNA so that destaining the background is not necessary with a low stain concentration (10 μg/ml). Description Ethidium bromide is a sensitive fluorescent stain for visualizing DNA in agarose and polyacrylamide gels. It is excited with a standard 302 nm UV transilluminator and emits a red-orange signal that can be photographed with Polaroid film or with a CCD-based gel documentation system.

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Ethidium bromide has been used in a number of fluorimetric assays for nucleic acids.15,16,17 It has been shown to bind to singlestranded DNA (although not as strongly)2 and triplestranded DNA.18 Because of the binding to DNA, EtBr is a powerful inhibitor of DNA polymerase.4 E7637, Molecular Biology grade powder, is suitable for use in gel electrophoresis and DNA isolation procedures. Ethidium bromide possesses UV absorbance maxima at 300 and 360 nm.

Ethidium bromide Revision Date 19-Jan-2018 9. Physical and chemical properties Physical State Powder Solid Appearance Purple Odor Slight Odor Threshold No information available pH 4.0-7.0 2% aq.sol Melting Point/Range 260 - 262 °C / 500 - 503.6 °F Boiling Point/Range No information available Flash Point > 100 °C / > 212 °F Ethidium bromide helps to identify DNA fragments, products of its enzymatic degradation that can be used in the PCR technique.
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ethidium bromide - Safety Data Sheet

red. Ethidium bromide. Definition på engelska: Agarose Gel Electrophoresis with Ethidium Bromide Staining. Andra betydelser av AGE/EBS.

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Etidium Svensk MeSH

Fragmenten analyserades på 1,2 % Agarosgel med 0,1 M TBE och 0,1 mg Ethidium bromide.